d cleanup kit bio rad laboratories  (Bio-Rad)

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ on native human saliva samples alter protein yield. Representaive 2D gel electrophoresis results of untreated ( a ) and ReadyPrep 2-D Cleanup kit™, ReadyPrep Reduction-Alkylation kit™ treated ( b ) native human saliva samples (no RNAlater™ treatment) demonstrate both procedures alter protein yield primarily at the low molecular range. Highlighted areas (1–3) indicate identical regions of the separation. Proteins were visualized by silver stain.

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Silver Staining

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Impact of reduction-alkylation during 2D gel electrophoresis of native saliva samples. Representative 2D gel electrophoresis results on human saliva samples with the use of ReadyPrep 2-D Cleanup kit™ ( a ) in comparison with ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ ( b ) treated samples indicate Reduction-Alkylation kit™ slightly improves spot detection and resolution. Arrowheads highlight visible differences in spot resolution and protein yield quality.

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Comparison

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Altered protein yields of RNAlater™ containing saliva samples following ReadyPrep 2D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ treatments in comparison with untreated, native saliva samples. 2D gel electrophoresis results of a representative untreated human saliva ( a ) compared with RNAlater™, ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ treated sample ( b ) indicate a significant decrease in protein yields despite of the protective manipulations. Highlighted areas (1–3) represent identical protein regions following separation.

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Comparison, Two-Dimensional Gel Electrophoresis, Electrophoresis

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Application of ReadyPrep Reduction-Alkylation kit™ does not improve the quality 2D gel electrophoresis of human saliva samples when pretreated with RNAlater™. Representative 2D gel electrophoresis of human selectively precipitated saliva samples with RNAlater™ ( a ) in relation with saliva sample treated with RNAlater™, ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ ( b ) demonstrate lack of significant separation improvement with RA (Black arrows point at identical proteins on a and b).

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis

Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Impact of RNAlater™ on 2D gel electrophoresis of saliva samples treated with both, ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™. The addition of RNAlater™ to precipitated and reduction-alkylated protein samples demonstrates a significant reduction of protein yield and quality following 2D separation ( b ) when compared to saliva without RNAlater™ ( a ). Numbers 1–3 correspond to similar regions of interest.

Article Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kitTM and ReadyPrep Reduction-Alkylation kitTM on native human saliva samples alter protein yield.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis

Journal: Plant Stress

Article Title: Proteomic profiling of an extreme halophyte Schrenkiella parvula with accelerated root elongation under mild salt stress.

doi: 10.1016/j.stress.2024.100357

Figure Lengend Snippet: Fig. 2. 2D gel electrophoresis analysis of differentially expressed proteins in Schrenkiella parvula root. Nine-day-old seedling roots (5 days after transfer to fresh 1/2 MS medium with or without 100 mM NaCl) were used for 2D gel electrophoresis. Proteins were isolated by the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio- Rad) and loaded on Bio-Rad ReadyStrip IPG Strips (pH 3–10 and pH 4–7) for separation based on their isoelectric point. The second separation of proteins via molecular weight was done using 10% SDS-PAGE. Proteins were visualized with Coomassie blue dye. A green plus symbol was used to indicate the position of the protein spots in the pictures.

Article Snippet: Extracted proteins were further purified using the Bio-Rad ReadyPrep 2-D Cleanup Kit (Bio-Rad).

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Isolation, Molecular Weight, SDS Page